Synthesis of optically active amino acids from alpha-keto acids with Escherichia coli cells expressing heterologous genes A Galkin, L Kulakova, T Yoshimura, K Soda, N Esaki Applied and environmental microbiology 63 (12), 4651-4656, 1997 | 214 | 1997 |
Pilot scale production and isolation of recombinant NAD+‐ and NADP+‐specific formate dehydrogenases VI Tishkov, AG Galkin, VV Fedorchuk, PA Savitsky, AM Rojkova, H Gieren, ... Biotechnology and bioengineering 64 (2), 187-193, 1999 | 154 | 1999 |
Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium Shewanella Strain Ac10: Gene Cloning and Enzyme Purification and Characterization L Kulakova, A Galkin, T Kurihara, T Yoshimura, N Esaki Applied and environmental microbiology 65 (2), 611-617, 1999 | 147 | 1999 |
Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly→ Pro substitution near the active site on its catalytic activity and … L Kulakova, A Galkin, T Nakayama, T Nishino, N Esaki Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1696 (1), 59-65, 2004 | 144 | 2004 |
Bacterial formate dehydrogenase. Increasing the enzyme thermal stability by hydrophobization of alpha-helices AM Rojkova, AG Galkin, LB Kulakova, AE Serov, PA Savitsky, ... FEBS letters 445 (1), 183-188, 1999 | 105 | 1999 |
Structural insight into arginine degradation by arginine deiminase, an antibacterial and parasite drug target A Galkin, L Kulakova, E Sarikaya, K Lim, A Howard, O Herzberg Journal of Biological Chemistry 279 (14), 14001-14008, 2004 | 101 | 2004 |
Crystal structures representing the Michaelis complex and the thiouronium reaction intermediate of Pseudomonas aeruginosa arginine deiminase A Galkin, X Lu, D Dunaway-Mariano, O Herzberg Journal of Biological Chemistry 280 (40), 34080-34087, 2005 | 88 | 2005 |
Catalytic properties and stability of a Pseudomonas sp. 101 formate dehydrogenase mutants containing Cys-255-Ser and Cys-255-Met replacements VI Tishkov, AG Galkin, GN Marchenko, OA Egorova, DV Sheluho, ... Biochemical and biophysical research communications 192 (2), 976-981, 1993 | 80 | 1993 |
Kinetic analysis of Pseudomonas aeruginosa arginine deiminase mutants and alternate substrates provides insight into structural determinants of function X Lu, L Li, R Wu, X Feng, Z Li, H Yang, C Wang, H Guo, A Galkin, ... Biochemistry 45 (4), 1162-1172, 2006 | 78 | 2006 |
Rational design of a trispecific antibody targeting the HIV-1 Env with elevated anti-viral activity JJ Steinhardt, J Guenaga, HL Turner, K McKee, MK Louder, S O’Dell, ... Nature communications 9 (1), 877, 2018 | 77 | 2018 |
Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10 A Galkin, L Kulakova, V Tishkov, N Esaki, K Soda Applied microbiology and biotechnology 44, 479-483, 1995 | 77 | 1995 |
Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10 A Galkin, L Kulakova, V Tishkov, N Esaki, K Soda Applied microbiology and biotechnology 44, 479-483, 1995 | 77 | 1995 |
Formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101: gene cloning and expression in Escherichia coli VI Tishkov, AG Galkin, GN Marchenko, YD Tsygankov, AM Egorov Biotechnology and applied biochemistry 18 (2), 201-207, 1993 | 71 | 1993 |
Arginine deiminase uses an active-site cysteine in nucleophilic catalysis of L-arginine hydrolysis X Lu, A Galkin, O Herzberg, D Dunaway-Mariano Journal of the American Chemical Society 126 (17), 5374-5375, 2004 | 70 | 2004 |
Characterization, kinetics, and crystal structures of fructose-1, 6-bisphosphate aldolase from the human parasite, Giardia lamblia A Galkin, L Kulakova, E Melamud, L Li, C Wu, P Mariano, ... Journal of biological chemistry 282 (7), 4859-4867, 2007 | 67 | 2007 |
Effect of single‐point mutations Phe41→ His and Phe143→ Glu on folding and catalytic properties of recombinant horseradish peroxidase expressed in E. coli IG Gazaryan, VV Doseeva, AG Galkin, VI Tishkov FEBS letters 354 (3), 248-250, 1994 | 66 | 1994 |
Structural basis for inactivation of Giardia lamblia carbamate kinase by disulfiram A Galkin, L Kulakova, K Lim, CZ Chen, W Zheng, IV Turko, O Herzberg Journal of Biological Chemistry 289 (15), 10502-10509, 2014 | 62 | 2014 |
Conversion of α-keto acids to D-amino acids by coupling of four enzyme reactions A Galkin, L Kulakova, H Yamamoto, K Tanizawa, H Tanaka, N Esaki, ... Journal of fermentation and bioengineering 83 (3), 299-300, 1997 | 56 | 1997 |
High-Throughput Giardia lamblia Viability Assay Using Bioluminescent ATP Content Measurements CZ Chen, L Kulakova, N Southall, JJ Marugan, A Galkin, CP Austin, ... Antimicrobial agents and chemotherapy 55 (2), 667-675, 2011 | 53 | 2011 |
Cold-adapted alanine dehydrogenases from two Antarctic bacterial strains: gene cloning, protein characterization, and comparison with mesophilic and thermophilic counterparts A Galkin, L Kulakova, H Ashida, Y Sawa, N Esaki Applied and environmental microbiology 65 (9), 4014-4020, 1999 | 50 | 1999 |