Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection NT Wirth, E Kozaeva, PI Nikel Microbial Biotechnology 13 (1), 233-249, 2020 | 134 | 2020 |
Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida DC Volke, L Friis, NT Wirth, J Turlin, PI Nikel Metabolic engineering communications 10, e00126, 2020 | 81 | 2020 |
Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways NL Bitzenhofer, L Kruse, S Thies, B Wynands, T Lechtenberg, J Rönitz, ... Essays in Biochemistry 65 (2), 319-336, 2021 | 64 | 2021 |
Cohesin SA2 is a sequence-independent DNA-binding protein that recognizes DNA replication and repair intermediates P Countryman, Y Fan, A Gorthi, H Pan, J Strickland, P Kaur, X Wang, J Lin, ... Journal of Biological Chemistry 293 (3), 1054-1069, 2018 | 58 | 2018 |
SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes E Martínez-García, S Fraile, E Algar, T Aparicio, E Velázquez, B Calles, ... Nucleic Acids Research 51 (D1), D1558-D1567, 2023 | 51 | 2023 |
Synthetic metabolism for biohalogenation A Cros, G Alfaro-Espinoza, A De Maria, NT Wirth, PI Nikel Current opinion in biotechnology 74, 180-193, 2022 | 36 | 2022 |
QurvE: user-friendly software for the analysis of biological growth and fluorescence data NT Wirth, J Funk, S Donati, PI Nikel Nature Protocols 18 (8), 2401-2403, 2023 | 33 | 2023 |
Conservation and divergence in nucleotide excision repair lesion recognition N Wirth, J Gross, HM Roth, CN Buechner, C Kisker, I Tessmer Journal of Biological Chemistry 291 (36), 18932-18946, 2016 | 30 | 2016 |
Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-cis, cis-muconate in engineered Pseudomonas putida NT Wirth, PI Nikel Chem Catalysis 1 (6), 1234-1259, 2021 | 27 | 2021 |
A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes NT Wirth, N Gurdo, N Krink, Ŕ Vidal-Verdú, S Donati, ... Metabolic Engineering 74, 83-97, 2022 | 26 | 2022 |
Engineering Pseudomonas putida KT2440 for the production of isobutanol R Nitschel, A Ankenbauer, I Welsch, NT Wirth, C Massner, N Ahmad, ... Engineering in Life Sciences 20 (5-6), 148-159, 2020 | 24 | 2020 |
Rapid genome engineering of Pseudomonas assisted by fluorescent markers and tractable curing of plasmids DC Volke, NT Wirth, PI Nikel Bio-protocol 11 (4), e3917-e3917, 2021 | 13 | 2021 |
Rapid probing of the reactivity of P450 monooxygenases from the CYP116B subfamily using a substrate-based method RJ Li, JH Xu, YC Yin, N Wirth, JM Ren, BB Zeng, HL Yu New Journal of Chemistry 40 (10), 8928-8934, 2016 | 13 | 2016 |
Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection. Microb Biotechnol 13: 233–249 NT Wirth, E Kozaeva, PI Nikel | 9 | 2020 |
Core and auxiliary functions of one-carbon metabolism in Pseudomonas putida exposed by a systems-level analysis of transcriptional and physiological responses J Turlin, Ň Puiggené, S Donati, NT Wirth, PI Nikel Msystems 8 (3), e00004-23, 2023 | 8 | 2023 |
Standardization of regulatory nodes for engineering heterologous gene expression: a feasibility study PI Nikel, I Benedetti, NT Wirth, V de Lorenzo, B Calles Microbial Biotechnology 15 (8), 2250-2265, 2022 | 8 | 2022 |
Anaerobic glucose uptake in Pseudomonas putida KT2440 in a bioelectrochemical system L Pause, A Weimer, NT Wirth, AV Nguyen, C Lenz, M Kohlstedt, ... Microbial Biotechnology 17 (1), e14375, 2024 | 5 | 2024 |
Recursive genome engineering decodes the evolutionary origin of an essential thymidylate kinase activity in Pseudomonas putida KT2440 NT Wirth, K Rohr, A Danchin, PI Nikel Mbio 14 (5), e01081-23, 2023 | 5 | 2023 |
Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-cis, cis-muconate in engineered Pseudomonas putida. Chem Catalysis 1: 1234–1259 NT Wirth, PI Nikel | 5 | 2021 |
Engineering Reduced-Genome Strains of Pseudomonas putida for Product Valorization NT Wirth, PI Nikel Minimal Cells: Design, Construction, Biotechnological Applications, 69-93, 2020 | 4 | 2020 |